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T0221

DNA Suspension Buffer, pH 8.0. DNase/RNase Tested, PCR Grade. 1mL Tubes, 50-Pack, Sterile.

Availability

Ships in 1 - 2 Days

Container Size

2mL

Item Count

50 Tube(s)

Price per Item

$173.00

Fill Volume

1 mL

T0221

DNA Suspension Buffer, pH 8.0. DNase/RNase Tested, PCR Grade. 1mL Tubes, 50-Pack, Sterile.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

DNA Suspension Buffer, pH 8.0, DNase/RNase Tested, PCR Grade is a buffering solution for resuspension, dilution, and storage of DNA samples. The pH 8.0 buffering system supports DNA integrity during handling, and chelation of divalent metal ions helps limit nuclease activity.

Each lot is tested for detectable DNase and RNase. The buffer is suitable for PCR setup and amplification workflows, as well as cloning, sequencing, and library preparation procedures.

  • 10mM Tris-HCl
  • 0.1mM Ethylenediaminetetraacetic acid (EDTA)
  • DNA Resuspension and Storage: Reconstitutes and stores plasmid DNA, gDNA, PCR products, and oligos at pH 8.0; 10 mM Tris-HCl with 0.1 mM EDTA helps stabilize DNA and limit nuclease activity.

  • PCR/qPCR Template Dilution: Prepares DNA templates and standards for PCR, qPCR, and ddPCR; DNase/RNase-tested, PCR-grade for reliable amplification.

  • NGS Library Handling: Resuspends and dilutes sequencing libraries and amplicons prior to QC and loading, maintaining DNA integrity.

  • Sanger Sequencing Preparation: Dilutes purified PCR products or plasmids to appropriate concentrations for capillary sequencing.

  • DNA Standard Curves and Quantification: Creates stable dilution series for Qubit/PicoGreen assays and qPCR standard curves.

  • DNA Ladders and Controls Reconstitution: Rehydrates DNA ladders and control DNAs for downstream analysis.

  • Cloning and Transformation Workflows: Stores and transports purified plasmids for downstream cloning, restriction analysis, and transformation while helping preserve DNA quality.

  • Post-Purification Sample Handling: Maintains DNA pH and integrity during short- and long-term storage at 4°C or −20°C.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
730 days
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