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T0223

DNA Suspension Buffer, pH 8.0. RNase/DNase Tested, PCR. 250mL, Sterile. (10mM Tris, 0.1mM EDTA)

Availability

Ships in 1 - 2 Days

Container Size

250mL

Item Count

1 Bottle(s)

Price per Item

$39.00

Fill Volume

250 mL

T0223

DNA Suspension Buffer, pH 8.0. RNase/DNase Tested, PCR. 250mL, Sterile. (10mM Tris, 0.1mM EDTA)

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

DNA Suspension Buffer, pH 8.0, is a buffered solution for resuspension, dilution, and storage of purified DNA in molecular biology workflows, including PCR. The pH 8.0 environment helps maintain DNA integrity during handling and downstream processing.

Formulated with a buffering agent and a chelating agent, it limits divalent cation availability that can contribute to nuclease activity. RNase/DNase tested to reduce the risk of nucleic acid degradation.

  • 10mM Tris-HCl
  • 0.1mM Ethylenediaminetetraacetic acid (EDTA)
  • DNA Resuspension: Rehydrates and dissolves plasmid or genomic DNA pellets after extraction or precipitation.

  • DNA Storage: Buffers and protects DNA at pH 8.0; EDTA chelates divalent cations to inhibit nucleases during short- and long-term storage.

  • PCR/qPCR Template Dilution: Dilutes DNA templates and standards for PCR while maintaining DNA stability with minimal EDTA carryover.

  • Sequencing Sample Preparation: Dilutes and stores DNA for Sanger or NGS workflows to preserve integrity prior to enzymatic steps.

  • Standard Curve Preparation: Prepares stable DNA standards for qPCR and fluorescence-based DNA quantification assays.

  • Silica Column Elution: Serves as a gentle elution buffer alternative for DNA recovery from spin columns or magnetic beads.

  • Plasmid Handling: Maintains plasmid DNA quality for downstream cloning and analysis.

  • Sample Transport: Stabilizes purified DNA during shipment and short-term handling.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
730 days
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