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T0227

DNA Suspension Buffer, pH 8.0. 100mL, Sterile. DNase/RNase Tested, PCR Grade.

Availability

Ships in 1 - 2 Days

Container Size

125mL

Item Count

1 Bottle(s)

Price per Item

$30.00

Fill Volume

100 mL

T0227

DNA Suspension Buffer, pH 8.0. 100mL, Sterile. DNase/RNase Tested, PCR Grade.

Quantity

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Description
Formulation
Common Applications
Quality Specifications
Storage

DNA Suspension Buffer, pH 8.0 (DNase/RNase Tested, PCR Grade) is a nuclease-controlled buffer for resuspension, dilution, and storage of purified DNA for downstream applications such as PCR, cloning, restriction digestion, and sequencing. The pH 8.0 buffering environment and a trace chelating component support nucleic acid stability during routine handling and sample preparation.

Each lot is tested to verify the absence of detectable DNase and RNase activity. The buffer is suitable for preparation of DNA standards, library workflows, and long-term DNA archiving under appropriate storage conditions.

  • 10mM Tris-HCl
  • 0.1mM Ethylenediaminetetraacetic acid (EDTA)
  • DNA Resuspension: Rehydrates and dissolves purified DNA pellets post-extraction or ethanol precipitation.

  • Plasmid Prep Elution: Resuspends plasmid DNA from Escherichia coli minipreps for downstream use.

  • DNA Storage: Provides pH 8.0 stability with 10 mM Tris-HCl and nuclease inhibition via 0.1 mM EDTA for short- to long-term DNA preservation.

  • PCR/qPCR/ddPCR Template Dilution: Maintains template integrity and compatibility with polymerases while minimizing nuclease activity.

  • Library Preparation and NGS Dilutions: Dilutes amplicons and libraries while protecting DNA from degradation without excessive EDTA carryover.

  • Sanger Sequencing Templates: Prepares and stabilizes DNA templates and purified PCR products for capillary sequencing.

  • Oligo and gDNA/Oligonucleotide Reconstitution: Reconstitutes lyophilized primers, gBlocks, and standards for consistent performance.

  • Restriction Digest Template Preparation: Stabilizes DNA prior to enzymatic digestion; low EDTA minimizes interference with Mg2+-dependent enzymes.

  • DNA Quantification Standards: Resuspends and dilutes standards for fluorometric or spectrophotometric assays.

  • Sample Transport and Handling: Protects DNA integrity during routine handling, freeze-thaw, and shipment.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
730 days
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