Western Blot Washing: Standard wash buffer for blots to reduce nonspecific binding during antibody incubations.

Western Blot Blocking: Base buffer for blocking solutions (with BSA or milk) to minimize background on membranes.

ELISA Plate Washing: Used as a wash buffer between incubation steps to reduce nonspecific interactions.

Antibody Dilution: Diluent for primary and secondary antibodies to maintain stability and reduce background.

Immunohistochemistry/Immunocytochemistry: Wash buffer for fixed tissues and cells to improve signal-to-noise.

Immunofluorescence: Gentle detergent-containing wash to preserve epitopes while limiting nonspecific binding.

Membrane Washing After Transfer: Rinsing PVDF or nitrocellulose membranes prior to blocking and probing.

General Rinse for Fixed Samples: Isotonic buffer for rinsing fixed cells or tissues during staining workflows.