Custom Order

We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.

Y5300

YPD Broth with 1M Sorbitol, 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$93.00

Fill Volume

1000 mL

COA
COA

Y5300

YPD Broth with 1M Sorbitol, 1000mL, Sterile.

Quantity

scroll

Description
Formulation
Common Applications
Storage

YPD Broth with 1 M Sorbitol is a rich, nonselective medium for the cultivation of yeasts, including Saccharomyces cerevisiae and other laboratory and industrial strains. The sorbitol component provides defined osmotic support suitable for handling osmotically sensitive cells during procedures such as transformation, spheroplast preparation, and cell wall stress studies.

The medium supplies a readily metabolizable carbon source along with complex nitrogen, vitamins, and growth factors from peptone and yeast extract. The 1 M sorbitol concentration maintains osmotic balance to help preserve cell integrity in workflows that involve transient cell wall perturbation or high-osmolarity conditions.

  • 2.0% Peptone
  • 1.0% Yeast Extract
  • 2.0% Glucose
  • 1M Sorbitol

  • Yeast Cultivation: Rich medium (2.0% Peptone, 1.0% Yeast Extract, 2.0% Glucose) for routine growth and propagation of yeast strains.

  • Post-Transformation Recovery: 1M Sorbitol provides osmotic stabilization for recovery after lithium acetate/PEG or electroporation of yeast.

  • Spheroplasting and Regeneration: Supports cell wall digestion and subsequent regeneration with osmotic support.

  • High-Osmolarity Stress Studies: Used to probe HOG pathway signaling and osmoadaptation in yeast.

  • Recombinant Protein Expression: Supports robust yeast growth for expression screens and small-scale production.

  • Selection and Maintenance of Plasmids: Suitable base medium for drug/auxotrophic selection and stable maintenance of plasmid-bearing yeast.

  • Yeast Two-Hybrid Workflows: Growth of bait/prey strains prior to selection on dropout plates.

  • Starter Cultures for Fermentation: Pre-culturing yeast inocula for downstream fermentation or bioprocess optimization.

  • Cell Wall Integrity Assays: Evaluating sensitivity to cell wall-perturbing agents under osmotic support.

Storage Temperature
Room Temperature
Shelf Life
730 days
Shows related citations for this product
Explore related products
Looking for a Certificate of Analysis?

Enter a product’s Lot Number to download the Certificate of Analysis (COA).

GET YOUR COA
We're custom by default

Everything we do is built around getting you exactly what you need, from formulation and filtration to packaging. Talk to our team about creating a custom product today.

Let's find your solution

Find out what’s possible

 

Talk to our consultants today to discover custom solutions that can help you achieve your goals.

 

Get in touch:

1.800.209.4488

Monday to Friday

8:00AM–5:00PM PT

Contact us button