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Terrific Broth Complete

Description:  1.2% Tryptone, 2.4% yeast extract,

0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4.
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.
Shipping Conditions:
  Ambient.
Storage:
 Room temperature.

Cat #                     Unit                  Price
T7062                  250 ml               $21.00
T7055                  450 ml               $20.06
T7060                 1000 ml              $26.25

Terrific Broth

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol.  100ml of 10 x phosphate buffer supplement  included separately.  Add 50ml of 10 x phosphate buffer to 450ml of terrific broth.  Add 100ml of 10 x phosphate buffer to 900ml of terrific broth.
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.
Shipping Conditions:
  Ambient.
Storage:
  Room temperature.

Cat #                     Unit                  Price
T7050                  450 ml               $26.42
T7000                  900 ml               $38.16

Terrific Broth with 1.6% Glycerol

Description:   1.2% Tryptone, 2.4% yeast extract,1.6% glycerol.  100ml of 10 x phosphate buffer supplement supplied separately (P7100).  Add 100ml of 10 x phosphate buffer to 900ml of terrific broth.
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.  Higher plasmid yields may be obtained than with standard terrific broth.
Shipping Conditions:
  Ambient.
Storage:
  Room temperature.

Cat #                     Unit                  Price
T7100                  900 ml               $39.62

Terrific Broth Ampicillin-100 with 1.6% Glycerol 

Description:   1.2% Tryptone, 2.4% yeast extract, 1.6% glycerol, 72mM K2HPO4, 17mM KH2PO4 with 50µg/ml ampicillin. 
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.  Higher plasmid yields may be obtained than with standard terrific broth. 
Shipping Conditions:
  Ambient.
Storage:
  Room temperature.

Cat #                     Unit                  Price
T7105                  900 ml               $42.18

10 x Phosphate Buffer Supplement

Description:  720mM K2HPO4, 170mM KH2PO4.
Applications:
  For use with terrific broths.  Add 50ml of buffer per 450ml of terrific broth.  Add 100ml of 10 x phosphate buffer to 900ml of terrific broth.
Shipping Conditions:
  Ambient.
Storage:
  Room temperature.

Cat #                     Unit                  Price
P7100                  100 ml               $17.87
P7500                  500 ml               $25.52
P7000                 1000 ml              $41.25

Terrific Broth 10X Concentrate

Description:  12% Tryptone, 24% yeast extract, 4.0% glycerol.  Supplied with 1 liter of 10 x phosphate buffer (P7000). 
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.  The 10x concentrate is convenient for large scale and ferment or cultures.
Shipping Conditions:
  Ambient.
Storage:
  Room temperature or refrigerate.

Cat #                     Unit                  Price
T7010                 1000 ml             $176.13

Terrific Broth with Ampicillin-50

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with 50µg/ml ampicillin.  
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7015                  900 ml               $39.59

Terrific Broth with Ampicillin-100

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with 100µg/ml ampicillin.  
Applications:
  Use to obtain high yields of plasmid DNA from E.coli.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7021                  900 ml               $39.59

Terrific Broth with Ampicillin-100, Streptomycin-50

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4.with 100µg/ml ampicillin and 50µg/ml streptomycin.
Applications:
  Use to obtain high yields of plasmid DNA from transformed E.coli that are resistant to both ampicillin and streptomycin.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7200                  900 ml               $38.86

Terrific Broth Ampicillin-50 Miniprep Culture Tubes

Description:  1.2% Tryptone, 2.4% yeast extract,  0.4% glycerol, 72mM K2HPO4, 17mM KH2PO4 with 50µg/ml ampicillin  in 13ml culture tubes.  Each  tube contains 3.0ml of broth.  Pack of 25 tubes in a reusable rack.
Applications:
  For plasmid DNA minipreps.  LB is packaged in snap-cap culture tubes that can be used to both grow cells and centrifuge them once they are grown.  Suitable for use with ampicillin resistant strains and strains harboring plasmids such as pBluescript, pGEM, pUC etc...
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7500                 25 pack              $51.36

Terrific Broth Ampicillin-100 Miniprep Culture Tubes

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, 17mM KH2PO4 with 100µg/ml ampicillin in 13ml culture tubes.  Each  tube contains 3.0ml of broth and phosphate buffer.  Rack of 25 tubes. 
Applications:
  For plasmid DNA minipreps.  LB is packaged in snap-cap culture tubes that can be used to both grow cells and centrifuge them once they are grown.  Suitable for use with ampicillin resistant strains and strains harboring plasmids such as pBluescript, pGEM, pUC etc...
Shipping Conditions:
Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7505                 25 pack              $51.36

Terrific Broth with Carbenicillin-50

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with 50µg/ml carbenicillin.
Applications:
  Used to obtain high yields of plasmid DNA from E.coli cultures.  Suitable for use with carbenicillin (ampicillin) resistant strains and strains harboring plasmids such as pBluescript, pGEM, pUC etc...
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7025                  900 ml               $39.59

Terrific Broth Carbenicillin-100 Miniprep Culture Tubes

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, 17mM KH2PO4 with 100µg/ml carbenicillin.   Each tube contains 3.0ml of broth.  Rack of 25 tubes in a reusable rack.
Applications:
  For plasmid DNA minipreps and the maintenance and propagation of carbenicillin (ampicillin) resistant strains.  Broth is supplied in snap-cap culture tubes that can be used to grow cells and centrifuge them once they are grown.  Suitable for use with carbenicillin (ampicillin) resistant strains and strains harboring plasmids such as pBluescript, pGEM, pUC etc...
Shipping Conditions:
Ambient.
Storage:
  Refrigerate.

Cat #                     Unit                  Price
T7510                 25 pack              $48.91

Terrific Broth with Carbenicillin-100

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with 100µg/ml carbenicillin. 
Applications:
  Used to obtain high yields of plasmid DNA from E.coli cultures.  Suitable for use with carbenicillin (ampicillin) resistant strains and strains harboring plasmids such as pBluescript, pGEM, pUC etc...
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate

Cat #                     Unit                  Price
T7030                  900 ml               $39.59

Terrific Broth with Chloramphenicol-50

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with 50µg/ml chloramphenicol. 
Applications:
  Used to obtain high yields of plasmid DNA from E.coli. cultures.  Suitable for use chloramphenicol resistant strains and strains harboring chlorr plasmids.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate

Cat #                     Unit                  Price
T7035                  900 ml               $39.59

Terrific Broth with Kanamycin-25

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with  25µg/ml kanamycin.  
Applications:
  Used to obtain high yields of plasmid DNA from E.coli. cultures.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate

Cat #                     Unit                  Price
T7040                  900 ml               $39.59

Terrific Broth with Streptomycin-50

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with  50µg/ml streptomycin.
Applications:
  Used to obtain high yields of plasmid DNA from E.coli. cultures.  Suitable for use streptomycin resistant strains and strains harboring strep  plasmids
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate

Cat #                     Unit                  Price
T7045                  900 ml               $37.70

Terrific Broth with Tetracycline-25

Description:  1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol, 72mM K2HPO4, and 17mM KH2PO4 with  25µg/ml tetracycline.
Applications:
  Used to obtain high yields of plasmid DNA from E.coli. cultures.  Suitable for use tetracycline resistant strains and strains harboring tetr plasmids.
Shipping Conditions:
  Ambient.
Storage:
  Refrigerate

Cat #                     Unit                  Price
T7070                  900 ml               $37.70

 
Terrific Broths
For many, Terrific Broth is the liquid media of choice for plasmid preps.  While cells grown in Terrific Broth grow slower than in LB the final cell density and plasmid yields are 2-4 fold higher.  It is recommended that cells grown in TB are allowed to grow for 24 hours to achieve high densities.
Other Bacterial Liquid Media
Yeast Growth and Selection Media
   
 
 
 
 
 
 
 
 
 
 

2290 Bert Dr.
Hollister, CA 95023
Phone: 831-637-1100
Fax: 831-637-2355
info@teknova.com